No articles match
Understanding how the dsb method works8 months ago
Load example package data | log transform | dsb step 1 removal of ambient noise | dsb step II Part I fitting single cell models to extract background | dsb Part II step II | How can I interpret the dsb values?
Normalizing ADTs for datasets without empty droplets with the dsb function ModelNegativeADTnorm1 years ago
Fast normalization for large datasets with or without empty drops1 years ago
End-to-end CITE-seq analysis workflow using dsb for ADT normalization and Seurat for multimodal clustering2 years ago
Table of Contents | Background and motivation | Installation and quick overview | Download public 10X Genomics data | Step 1 A note on alignment of ADTs | Step 2 Load RNA and ADT data and define droplet quality control metadata | Step 3 Quality control cells and background droplets | Optional step; remove proteins without staining | Step 4 Normalize protein data with the DSBNormalizeProtein Function | Integrating dsb with Seurat | Clustering cells based on dsb normalized protein using Seurat | dsb derived cluster interpretation | Weighted Nearest Neighbor multimodal clustering using dsb normalized values with Seurat | Method 1 -- Seurat WNN default with PCA on dsb normalized protein | Method 2-- Seurat WNN with dsb normalized protein directly without PCA | Some recent publications using dsb | using other alignment algorithms | A note on Cell Ranger --expect-cells
Additional Topics - qualtile.clipping - scale.factor - Python and Bioc - multiplexing - multi batch - FAQ3 years ago
Integrating dsb with Bioconductor | Using dsb in Python | Using dsb with data lacking isotype controls | Using dsb with sample multiplexing experiments | Advanced usage - return internal stats used by dsb | outlier clipping with the quantile.clipping argument | Using a different background scaling method | Frequently Asked Questions | check for outliers in dsb normalized values
HDStIM4 years ago
To Run The Main HDStIM Function | Output | To Plot Diagnostic Figures | Plots Explaining K-means Clustering And Fisher's Exact Test | UMAP Plots To Visually Inspect Responding and Non-Responding Cell Mapping | Distribution Plots for Individual State Marker before And After Mapping | To Rank State/Signaling Markers According To Their Contribution To The Response