End-to-end CITE-seq analysis workflow using dsb for ADT normalization and Seurat for multimodal clustering2 years ago
Table of Contents | Background and motivation | Installation and quick overview | Download public 10X Genomics data | Step 1 A note on alignment of ADTs | Step 2 Load RNA and ADT data and define droplet quality control metadata | Step 3 Quality control cells and background droplets | Optional step; remove proteins without staining | Step 4 Normalize protein data with the DSBNormalizeProtein Function | Integrating dsb with Seurat | Clustering cells based on dsb normalized protein using Seurat | dsb derived cluster interpretation | Weighted Nearest Neighbor multimodal clustering using dsb normalized values with Seurat | Method 1 -- Seurat WNN default with PCA on dsb normalized protein | Method 2-- Seurat WNN with dsb normalized protein directly without PCA | Some recent publications using dsb | using other alignment algorithms | A note on Cell Ranger --expect-cells
dsb 2.0.1Matt Mulèend_to_end_workflow.rmd